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Western blot
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- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB275-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate CXCL1/GRO alpha/KC/CINC-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL1/GRO alpha/KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr)CINC-1, rrCINC-2 alpha , rrCINC-2 beta , rrCINC-3, rhMIP-1 alpha , recombinant mouse (rm)MIP-1 alpha , rmMIP-1 beta , rmMIP-1 beta , rhMIP-1 delta , rmMIP-1 gamma , rmMIP-2, rhMIP-3 alpha , rmMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , or rmMIP-3 beta .
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 20326
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CXCL1 induces senescence of cancer-associated fibroblasts via autocrine loops in oral squamous cell carcinoma.
Tumor microenvironment in functional adrenocortical adenomas: immune cell infiltration in cortisol-producing adrenocortical adenoma.
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells.
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
The expression and prognostic impact of CXC-chemokines in stage II and III colorectal cancer epithelial and stromal tissue.
Protein chip discovery of secreted proteins regulated by the phosphatidylinositol 3-kinase pathway in ovarian cancer cell lines.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells.
Kim EK, Moon S, Kim DK, Zhang X, Kim J
PloS one 2018;13(1):e0188847
PloS one 2018;13(1):e0188847
Tumor microenvironment in functional adrenocortical adenomas: immune cell infiltration in cortisol-producing adrenocortical adenoma.
Kitawaki Y, Nakamura Y, Kubota-Nakayama F, Yamazaki Y, Miki Y, Hata S, Ise K, Kikuchi K, Morimoto R, Satoh F, Sasano H
Human pathology 2018 Jul;77:88-97
Human pathology 2018 Jul;77:88-97
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Logue SE, McGrath EP, Cleary P, Greene S, Mnich K, Almanza A, Chevet E, Dwyer RM, Oommen A, Legembre P, Godey F, Madden EC, Leuzzi B, Obacz J, Zeng Q, Patterson JB, Jäger R, Gorman AM, Samali A
Nature communications 2018 Aug 15;9(1):3267
Nature communications 2018 Aug 15;9(1):3267
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells.
Chan TS, Hsu CC, Pai VC, Liao WY, Huang SS, Tan KT, Yen CJ, Hsu SC, Chen WY, Shan YS, Li CR, Lee MT, Jiang KY, Chu JM, Lien GS, Weaver VM, Tsai KK
The Journal of experimental medicine 2016 Dec 12;213(13):2967-2988
The Journal of experimental medicine 2016 Dec 12;213(13):2967-2988
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam TV, Leypoldt F, Simova O, Thom V, Friese MA, Prinz I, Hölscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
Blood 2012 Nov 1;120(18):3793-802
Blood 2012 Nov 1;120(18):3793-802
Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Meen AJ, Øynebråten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
The Journal of biological chemistry 2011 Jan 28;286(4):2636-47
The Journal of biological chemistry 2011 Jan 28;286(4):2636-47
The expression and prognostic impact of CXC-chemokines in stage II and III colorectal cancer epithelial and stromal tissue.
Oladipo O, Conlon S, O'Grady A, Purcell C, Wilson C, Maxwell PJ, Johnston PG, Stevenson M, Kay EW, Wilson RH, Waugh DJ
British journal of cancer 2011 Feb 1;104(3):480-7
British journal of cancer 2011 Feb 1;104(3):480-7
Protein chip discovery of secreted proteins regulated by the phosphatidylinositol 3-kinase pathway in ovarian cancer cell lines.
Moscova M, Marsh DJ, Baxter RC
Cancer research 2006 Feb 1;66(3):1376-83
Cancer research 2006 Feb 1;66(3):1376-83
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells.
Hagiwara H, Mitsumata M, Yamane T, Jin X, Yoshida Y
Circulation 1998 Dec 8;98(23):2584-90
Circulation 1998 Dec 8;98(23):2584-90
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCL1/GRO alpha/KC/CINC-1 in Human PBMCs Treated with LPS and Monensin by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) treated with 1 μg/mL LPS for 24 hours and 3 μM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GRO alpha/ KC/CINC-1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody. Recombinant Human CXCL1/GRO alpha/KC/CINC-1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GRO alpha/KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275). The ND50 is typically 3-15 µg/mL.
