50-7515-42
antibody from Invitrogen Antibodies
Targeting: CXCL1
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-7515-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL1 (GRO alpha) Monoclonal Antibody (KTYFLF), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This KTYFLF monoclonal antibody reacts with human CXCL1 and has no cross-reactivity to human CXCL2 or CXCL3. CXCL1, or GRO (growth-related oncogene) alpha, is a pro-inflammatory CXC chemokine first identified by its constitutive overexpression in some tumors. It is closely related to CXCL2 (GRO beta) and CXCL3 (GRO gamma), with which it shares 90% and 86% sequence homology, respectively. These proteins, along with IL-8 or CXCL8, were later found to be critical for neutrophil mobilization and degranulation, as well as vascular permeabilization and angiogenesis. Signaling occurs through the G protein-coupled receptor CXCR2, which is shared with GRO beta and gamma. CXCR2 can also be activated by IL-8, although some studies suggest that the majority of IL-8 activity on neutrophils is mediated by CXCR1. CXCL1 is secreted by monocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli such as LPS, IL-1 beta, and TNF alpha. Overexpression is observed in many malignant tumors, where it contributes to tumor vascularization and metastasis. Applications Reported: This KTYFLF antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This KTYFLF antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of peripheral blood monocytes. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- KTYFLF
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references PECAM1 Combines With CXCR4 to Trigger Inflammatory Cell Infiltration and Pulpitis Progression Through Activating the NF-κB Signaling Pathway.
Opening the flood-gates: how neutrophil-endothelial interactions regulate permeability.
Impaired interleukin-8- and GROalpha-induced phosphorylation of extracellular signal-regulated kinase result in decreased migration of neutrophils from patients with myelodysplasia.
Role of CXCL1 in tumorigenesis of melanoma.
Liu Y, Zhang Z, Li W, Tian S
Frontiers in cell and developmental biology 2020;8:593653
Frontiers in cell and developmental biology 2020;8:593653
Opening the flood-gates: how neutrophil-endothelial interactions regulate permeability.
DiStasi MR, Ley K
Trends in immunology 2009 Nov;30(11):547-56
Trends in immunology 2009 Nov;30(11):547-56
Impaired interleukin-8- and GROalpha-induced phosphorylation of extracellular signal-regulated kinase result in decreased migration of neutrophils from patients with myelodysplasia.
Fuhler GM, Knol GJ, Drayer AL, Vellenga E
Journal of leukocyte biology 2005 Feb;77(2):257-66
Journal of leukocyte biology 2005 Feb;77(2):257-66
Role of CXCL1 in tumorigenesis of melanoma.
Dhawan P, Richmond A
Journal of leukocyte biology 2002 Jul;72(1):9-18
Journal of leukocyte biology 2002 Jul;72(1):9-18
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood monocytes were unstimulated (left) or stimulated with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were fixed and stained intracellularly with Anti-Human CD14 PE (Product # 12-0149-42) and Anti-Human CXCL1 eFluor® 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Cells in the monocyte gate were used for analysis.