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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-41996 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: DRAEQAEADK KAAEDRSKQL EDELVSLQKK LKGTEDELDK YSEALKDAQE
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 cell lysate using an anti-Tropomyosin 1 polyclonal antibody (Product # PA5-41996). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-Tropomyosin 1 polyclonal antibody (Product # PA5-41996).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of U-2 OS (Lane 1), A549 (Lane 2), C2C12 (Lane 3), K-562 (Lane 4), HL-60 (Lane 5), Raji (Lane 6), tissue extracts of Mouse Skeletal Muscle (Lane 7 ) and Rat Skeletal Muscle (Lane 8). The blot was probed with Anti-TPM1 Polyclonal Antibody (Product # PA5-41996, 1.25µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to TPM1 was observed in U-2 OS, A549 and tissue extracts tested except K-562, HL-60, Raji which are reported negative for TPM1 expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human muscle tissue using an anti-Tropomyosin 1 polyclonal antibody (Product # PA5-41996).
