MA1097

antibody from Boster Biological Technology

Targeting: TPM1 C15orf13, CMH3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1097

Provider

Boster Biological Technology

Product name

Anti-Tropomyosin(Sarcomeric) Antibody (monoclonal)

Antibody type

Monoclonal

Antigen

Chicken muscle tropomyosin.

Description

Ascites

Reactivity

Human, Rat, Chicken/Avian

Vial size

100μg/vial

Concentration

Lyophilized

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human Caco-2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tropomyosin(Sarcomeric) antigen affinity purified monoclonal antibody (Catalog # MA1097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(Sarcomeric) at approximately 39KD. The expected band size for Tropomyosin(Sarcomeric) is at 33KD.

Additional image