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Learn200-301-F35
antibody from Rockland Immunochemicals, Inc.
Targeting: HSPA9
GRP75, HSPA9B, mot-2, mthsp75, PBP74
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-F35 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-F35, RRID:AB_2611157
- Product name
- Anti-Grp75 (MOUSE) Monoclonal Antibody - 200-301-F35
- Antibody type
- Monoclonal
- Description
- Anti-Grp75 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Grp75 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Grp75 from other sources has not been determined. Heat Shock research.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- S52A-42
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse anti-Grp75 antibody. Lane 1: HeLa Cell lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: Grp75 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 73.7 kDa, ~75 kDa for Grp75. Other band(s): none.
- Validation comment
- Western Blot
