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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-35315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transthyretin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transthyretin amyloid fibrils alter primary fibroblast structure, function, and inflammatory gene expression.
Dittloff KT, Iezzi A, Zhong JX, Mohindra P, Desai TA, Russell B
American journal of physiology. Heart and circulatory physiology 2021 Jul 1;321(1):H149-H160
American journal of physiology. Heart and circulatory physiology 2021 Jul 1;321(1):H149-H160
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Transthyretin in Human pancreas tissues and Human kidney tissues. Samples were incubated with Transthyretin polyclonal antibody (Product # PA5-35315) using a dilution of 0.0590277777777778 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG at a dilution of 1:400 (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Transthyretin in paraformaldehyde-fixed, paraffin-embedded human liver tissue sections. Samples were incubated with Transthyretin polyclonal antibody (Product # PA5-35315) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TTR in A375 cells (right) compared to a negative control (left) using a TTR polyclonal antibody (Product # PA5-35315) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
