PA5-27220

antibody from Invitrogen Antibodies

Targeting: TTR CTS, CTS1, HsT2651, PALB

Antibody data

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Validation data

Product number: PA5-27220 - Provider product page
Provider: Invitrogen Antibodies
Product name: Transthyretin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: Human plasma, Mouse liver, rat plasma. Predicted reactivity: Mouse (81%), Rat (82%), Cat (85%), Pig (87%), Sheep (85%), Rhesus Monkey (93%), Chimpanzee (97%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.58 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TTR protein structure - PA5-27220 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transthyretin in Human tissue extract (30 µg). Samples was separated by 15% SDS-PAGE and the membrane was probed with Transthyretin Polyclonal antibody (Product # PA5-27220) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transthyretin using 50 µg of mouse liver lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Transthyretin polyclonal antibody (Product # PA5-27220) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transthyretin was performed by separating 30 µg of human plasma by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Transthyretin Polyclonal Antibody (Product # PA5-27220) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transthyretin was performed by separating 50 µg of rat plasma by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Transthyretin Polyclonal Antibody (Product # PA5-27220) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Transthyretin Polyclonal Antibody (Product # PA5-27220). Mouse plasma (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Transthyretin Polyclonal Antibody (Product # PA5-27220) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Transthyretin Polyclonal Antibody (Product # PA5-27220) and a 15 kDa band along with lower band as reported isoform and an uncharacterized band (*) at ~80 kDa corresponding to Transthyretin was observed in plasma sample, 2 µL lysate of Human Plasma (Lane 1) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 Immunohistochemistry in the postischemic brain. Scattered cells were immunoreactive for TTR in the IC and PI 14 days after ischemic stroke. Low magnification overview of the ischemic territory using 1:1000 anti-TTR rabbit antibody (ThermoScientific cat# PA5-27220, Rockford, USA) (A) without and (D) with BP. (B, E and E') Higher magnification micrographs of images (A) showing cells immunoreactivity for TTR (white arrows). (C) Epithelial cells from CP were positive for TTR and (F) partially blocked with BP. (G) Staining for TTR (green, AF488) and with higher magnification in (J) together with DAPI (H). (J) Epithelial cells from CP from the same section. Scale bars: A , C , D and F-- 100 mum, B and E-- 20 mum and E' - 10 mum. BP-Blocking peptide; CP--Choroid plexus; IC--Infarct core; PI-Peri-infarct area; TTR-Transthyretin; LV-Lateral ventricle.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5 Evaluation of a chicken TTR antibody and TTR levels in brain parenchyma. (A) In contrast to protein extracts obtained from ischemic territory (infarct core-peri-infarct area) at 24 hours, 48 hours, 7 days and 14 days after PT, the monomeric form of TTR is found in samples of mouse serum, used as positive control. (B) TTR in human and mouse serum and absence of the protein in the cortical ischemic territory 14 days after PT. Instead, a band of approximately 60 to 65 kDa is found, not corresponding to the TTR protein (C) Blocking of specific TTR bands by preincubation with a specific TTR peptide. (D) Western blot performed without primary antibody incubation. HS-Human serum; M-Marker; MS-mouse serum; PT-Photothrombosis; TTR-Transthyretin; cx-cortex.