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LearnTA809279
antibody from OriGene
Targeting: MFGE8
BA46, EDIL1, hP47, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA809279 - Provider product page
- Provider
- OriGene
- Product name
- MFGE8 mouse monoclonal antibody,clone OTI7A4
- Antibody type
- Monoclonal
- Description
- MFGE8 mouse monoclonal antibody,clone OTI7A4
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MFGE8
- Isotype
- IgG
- Antibody clone number
- OTI7A4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MFGE8 (RC217163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MFGE8.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MFGE8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809279)(1:500)
- Validation comment
- IHC
