MAB2767
antibody from R&D Systems
Targeting: MFGE8
BA46, EDIL1, hP47, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB2767 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MFG-E8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MFG-E8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) C1r, rhC1s, rhC2, or rmMFG-E8.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q08431
- Isotype
- IgG
- Antibody clone number
- 278901
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model.
Lin YP, Hsu ME, Chiou YY, Hsu HY, Tsai HC, Peng YJ, Lu CY, Pan CY, Yu WC, Chen CH, Chi CW, Lin CH
Proteomics 2010 Jul;10(13):2429-43
Proteomics 2010 Jul;10(13):2429-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MFG-E8 by Simple WesternTM. Simple Western lane view shows recombinant human (rh) MFG-E8, loaded at 50 ng/mL, and human milk, loaded at 0.2 mg/mL. A specific band was detected for MFG-E8 at approximately 57-58 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human MFG-E8 Monoclonal Antibody (Catalog # MAB2767) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MFG-E8 by Western Blot. Western blot shows lysates of human milk. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MFG-E8 Monoclonal Antibody (Catalog # MAB2767) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.