PA1-12541

antibody from Invitrogen Antibodies

Targeting: CX3CR1 CCRL1, CMKBRL1, CMKDR1, GPR13, V28

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12541

Provider

Invitrogen Antibodies

Product name

CX3CR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for this product is human spleen lysate.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CX3CR1 Polyclonal Antibody (Product # PA1-12541) and ~52kDa band corresponding to CX3CR1 was observed across cell lines tested along with an uncharacterized band at ~42kDa. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), SK-O-V3 (Lane 2), SH-SY5Y (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).