600-401-493

antibody from Rockland Immunochemicals, Inc.

Targeting: ATF3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-493

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-493, RRID:AB_2058584

Product name

Anti-ATF3 (RABBIT) Antibody - 600-401-493

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells.

Xie JJ, Xu LY, Wu JY, Shen ZY, Zhao Q, Du ZP, Lv Z, Gu W, Pan F, Xu XE, Xie D, Li EM
The American journal of pathology 2010 Feb;176(2):939-51

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Rockland's Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Validation comment

Western Blot