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LearnPA5-23216
antibody from Invitrogen Antibodies
Targeting: DACT1
DAPPER, DAPPER1, FRODO, HDPR1, THYEX3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-23216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DACT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A transcriptional and post-transcriptional dysregulation of Dishevelled 1 and 2 underlies the Wnt signaling impairment in type I Gaucher disease experimental models.
Costa R, Bellesso S, Lualdi S, Manzoli R, Pistorio V, Filocamo M, Moro E
Human molecular genetics 2020 Jan 15;29(2):274-285
Human molecular genetics 2020 Jan 15;29(2):274-285
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DACT1 in 0.5 µg/mL brain lysate. Samples were incubated in DACT1 polyclonal antibody (Product # PA5-23216 using a dilution of 5 µg/mL. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show Dact1 antibody and rat brain lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DACT1 in formalin-fixed paraffin-embedded human brain. Samples were incubated in DACT1 polyclonal antibody (Product # PA5-23216) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
