GTX15789

antibody from GeneTex

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX15789

Provider

GeneTex

Product name

STAT3 antibody [9D8]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

References

Submitted references

Targeted regulationof STAT3 by miR-29a in mediating Taxol resistance of nasopharyngeal carcinoma cell line CNE-1.

Gao J, Shao Z, Yan M, Fu T, Zhang L, Yan Y
Cancer biomarkers : section A of Disease markers 2018;22(4):641-648

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of STAT3 in 25ug of various whole cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with STAT3 antibody [9D8] at a dilution of 1:5000 overnight at 4¢XC on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of STAT3 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with STAT3 antibody [9D8] at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488-conjugated secondary antibody. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of STAT3 from HepG2 cell lysate. The antigen-antibody complex was formed by incubating 750 £gg of whole cell lysate with 2 £gg of STAT3 antibody [9D8] overnight on a rocking platform at 4¢XC. The immune-complex was then captured on 50 £gl Protein A/G Agarose. Captured immune-complexes were then washed extensively and eluted. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with STAT3 antibody [9D8] at a dilution of 1:5000 overnight rotating at 4¢XC. Membranes were washed in TBST and probed with a proper secondary antibody. Membranes were washed and chemiluminescent detection was performed.