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- Other assay [1]
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- Product number
- 701062 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT3 (Tyr705) Recombinant Rabbit Monoclonal Antibody (3H16L22)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3H16L22
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-STAT3 pTyr705 in whole cell extracts of HeLa treated with IFN1a (150 ng/mL, 15 min, lane 1 and 3) or with Insulin (100 ng/mL, 15 min, lane 2 and 4) using a Phospho-STAT3 pTyr705 recombinant rabbit monoclonal antibody (Product # 701062) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lanes 3 and 4). Results show a band at ~90 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Indirect ELISA analysis of endogenous Phospho-STAT3 pTyr705 in HeLa cells (treated with Insulin) coated onto the plate using a Phospho-STAT3 pTyr705 recombinant rabbit monoclonal antibody (Product # 701062) at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.
