Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- STJ97525 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-STAT3 antibody [3F4] (STJ97525)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Signal Transducer And Activator Of Transcription 3 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of STAT3 in human breast cancer cell line MCF-7 (A), T47D (B), MDA-MB-231 (C) and Cal51 (D) using STAT3 mouse mAb ( (STJ97525, 1:2000 diluted).Predicted band size: 86kDa.Observed band size:86kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of 1) Rat Heart Tissue, 2) Mouse Heart Tissue, 3) Hela with STAT3 Mouse mAb diluted at 1:2, 000.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using STAT3Mouse mAb diluted at 1:200.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using STAT3Mouse mAb diluted at 1:200.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA