PAB29373

antibody from Abnova Corporation

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29373

Provider

Abnova Corporation

Product name

STAT3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human STAT3.

Antigen sequence

GVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAE
IIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYC
RPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTI
DLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTF
DMELTSECA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with STAT3 polyclonal antibody (Cat # PAB29373) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with STAT3 polyclonal antibody (Cat # PAB29373) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lung with STAT3 polyclonal antibody (Cat # PAB29373) shows distinct nuclear positivity in pneumocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)