H00006774-M01

antibody from Abnova Corporation

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006774-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006774-M01, RRID:AB_425694

Product name

STAT3 monoclonal antibody (M01), clone 1D11-2A11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant STAT3.

Antigen sequence

MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLA
PWIESQDWAYAASKESHATLVFHNLLGEIDQQYSR
FLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIV
ARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEK
QQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFN
YKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTAL
DQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKR
RQQIACIGGPPNICLDRLENWITSLAESQLQTRQQ
IKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRN
LMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVR
LLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFN
ILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGN
GGRANCDASLIVTEELHLITFETEVYHQGLKIDLE
THSLPVVVISNICQMPNAWASILWYNMLTNNPKNV
NFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQL
TTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFS
FWVWLDNIIDLVKKYILALWNEGYIMGFISKERER
AILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISG
KTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNI
LVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
AAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQ
FGNNGEGAEPSAGGQFESLTFDMELTSECATSPM


Isotype

IgG

Antibody clone number

1D11-2A11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STAT3 monoclonal antibody (M01), clone 1D11-2A11 Western Blot analysis of STAT3 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STAT3 monoclonal antibody (M01), clone 1D11-2A11. Western Blot analysis of STAT3 expression in K-562 ( Cat # L009V1 ).

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between STAT1 and STAT3. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-STAT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)