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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB10397 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10397, RRID:AB_11189541
- Product name
- STAT3 monoclonal antibody, clone 3B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant STAT3.
- Isotype
- IgG
- Antibody clone number
- 3B5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) against HeLa (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS-7 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue (A) and lung cancer tissue (B) using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
