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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- MA5-15193 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT3 (Tyr705) Monoclonal Antibody (R.263.6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- R.263.6
- Vial size
- 100 µL
- Concentration
- 65 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-STAT3 pTyr705 in extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/mL), using Phospho-STAT3 pTyr705 monoclonal antibody (Product # MA5-15193).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hela treated with IFNa (150ng/ml for 15 minutes) (Lane 2), Jurkat (Lane 3) and Jurkat treated with IFNa (150ng/ml for 15 minutes) (Lane 4). The blot was probed with Anti-Phospho-STAT3 (Tyr705) Monoclonal Antibody (R.263.6) (Product # MA5-15193, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). An 88 kDa band corresponding to Phospho-STAT3 (Tyr705) was observed to appear upon treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Phospho-STAT3 pTyr705 in untreated (red) or IFN-alpha-treated (blue) NIH/3T3 cells using a Phospho-STAT3 pTyr705 monoclonal antibody (Product # MA5-15193).
