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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- NB100-799 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-799, RRID:AB_2271358
- Product name
- Goat Polyclonal STAT3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
DMELTSECATSPM- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Stat3 as an oncogene.
Stat3 as an oncogene.
Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr
Cell 1999 Aug 6;98(3):295-303
Cell 1999 Aug 6;98(3):295-303
Stat3 as an oncogene.
Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr
Cell 1999 Aug 6;98(3):295-303
Cell 1999 Aug 6;98(3):295-303
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - (4ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Staining of Mouse Thymus. Antibody at 4 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Negative Control showing staining of Mouse Thymus, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: STAT3 Antibody [NB100-799] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
