Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4319 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STAT4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human STAT4 when phosphorylated at Y693.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.
Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
Yu SF, Zhang YN, Yang BY, Wu CY
The Journal of biological chemistry 2014 Dec 19;289(51):35561-9
The Journal of biological chemistry 2014 Dec 19;289(51):35561-9
Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
Wang X, Bishop KA, Hegde S, Rodenkirch LA, Pike JW, Gumperz JE
The Journal of experimental medicine 2012 May 7;209(5):987-1000
The Journal of experimental medicine 2012 May 7;209(5):987-1000
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Phospho-STAT4 (Y693) by Western Blot. Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.