Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA-ST4 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STAT4 Antibody
- Antibody type
- Polyclonal
- Description
- Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 200 uL
- Storage
- Store the unopened product at -20 to -70 degreesC. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Submitted references Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
Liao W, Lin JX, Wang L, Li P, Leonard WJ
Nature immunology 2011 Jun;12(6):551-9
Nature immunology 2011 Jun;12(6):551-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 106 - 107 cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.
