Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00006775-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006775-M01, RRID:AB_548418
- Product name
- Mouse Monoclonal STAT4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. STAT4 - signal transducer and activator of transcription 4
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of STAT4 expression in transfected 293T cell line by STAT4 monoclonal antibody (M01), clone 1C2-1C12.Lane 1: STAT4 transfected lysate(85.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - STAT4 monoclonal antibody (M01), clone 1C2-1C12. Analysis of STAT4 expression in human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - STAT4 monoclonal antibody (M01), clone 1C2-1C12 Analysis of STAT4 expression in A-549.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Detection limit for recombinant GST tagged STAT4 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of monoclonal antibody to STAT4 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of STAT4 transfected lysate using anti-STAT4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAT4 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of monoclonal antibody to STAT4 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 1 ~ 10 ug/ml.