Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-9044-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT4 (Tyr693) Monoclonal Antibody (4LURPIE), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells. Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry. Applications Reported: This 4LURPIE antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4LURPIE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4LURPIE
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.
Interleukin-10 production by Th1 cells requires interleukin-12-induced STAT4 transcription factor and ERK MAP kinase activation by high antigen dose.
IFN-alpha is not sufficient to drive Th1 development due to lack of stable T-bet expression.
Distinct characteristics of murine STAT4 activation in response to IL-12 and IFN-alpha.
Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages.
Hu M, Lu Y, Qi Y, Zhang Z, Wang S, Xu Y, Chen F, Tang Y, Chen S, Chen M, Du C, Shen M, Wang F, Su Y, Deng Y, Wang J
Cancer immunology research 2020 Sep;8(9):1150-1162
Cancer immunology research 2020 Sep;8(9):1150-1162
Interleukin-10 production by Th1 cells requires interleukin-12-induced STAT4 transcription factor and ERK MAP kinase activation by high antigen dose.
Saraiva M, Christensen JR, Veldhoen M, Murphy TL, Murphy KM, O'Garra A
Immunity 2009 Aug 21;31(2):209-19
Immunity 2009 Aug 21;31(2):209-19
IFN-alpha is not sufficient to drive Th1 development due to lack of stable T-bet expression.
Ramos HJ, Davis AM, George TC, Farrar JD
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3792-803
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3792-803
Distinct characteristics of murine STAT4 activation in response to IL-12 and IFN-alpha.
Berenson LS, Gavrieli M, Farrar JD, Murphy TL, Murphy KM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5195-203
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5195-203
Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages.
Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM, Weaver CT
Nature immunology 2005 Nov;6(11):1123-32
Nature immunology 2005 Nov;6(11):1123-32
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of untreated (orange histogram) or 15-minute IFN alpha2c-treated (purple histogram) Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81)-activated human CD4+ T cells with Anti-Human/Mouse phospho-STAT4 (Y693) APC. Cells in the lymphocyte gate were used for analysis.