TA502940

antibody from OriGene

Targeting: STAT4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502940

Provider

OriGene

Proper citation

OriGene Cat#TA502940, RRID:AB_11129196

Product name

STAT4 mouse monoclonal antibody, clone OTI4H6 (formerly 4H6)

Antibody type

Monoclonal

Description

STAT4 mouse monoclonal antibody, clone OTI4H6 (formerly 4H6)

Host

Mouse

Conjugate

Unconjugated

Epitope

STAT4

Isotype

IgG

Antibody clone number

OTI4H6

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT4 (RC206892 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT4.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-STAT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502940)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-STAT4 antibody(TA502940),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206892 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-STAT4 antibody(TA502940), and then analyzed by flow cytometry.

Validation comment

FC