MA5-14976

antibody from Invitrogen Antibodies

Targeting: STAT4

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-14976

Provider

Invitrogen Antibodies

Product name

STAT4 Monoclonal Antibody (H.56.9)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

H.56.9

Vial size

100 µL

Storage

-20°C

References

Submitted references

The Minor Allele of rs7574865 in the STAT4 Gene Is Associated with Increased mRNA and Protein Expression.

Lamana A, López-Santalla M, Castillo-González R, Ortiz AM, Martín J, García-Vicuña R, González-Álvaro I
PloS one 2015;10(11):e0142683

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-STAT4 Monoclonal Antibody (H.56.9) (Product # MA5-14976) and a 86kDa band corresponding to Signal transducer and activator of transcription 4 was observed across cell lines and tissues tested . Whole cell extracts (50 µg lysate) of Jurkat (Lane 1), HEK-293 (Lane 2), A549 (Lane 3), HeLa (Lane 4), Daudi (Lane 5), HL-60 (Lane 6), Raji (Lane 7), MCF7 (Lane 8), Mouse Testis (Lane 9), Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Relative expression was observed in cell lines as well as in tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Stat4 in extracts from ACHN and SR cell lines using Stat4 monoclonal antibody (Product # MA5-14976).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-STAT4 Monoclonal Antibody (H.56.9) (Product # MA5-14976) and a 86kDa band corresponding to Signal transducer and activator of transcription 4 was observed across cell lines and tissues tested . Whole cell extracts (50 µg lysate) of Jurkat (Lane 1), HEK-293 (Lane 2), A549 (Lane 3), HeLa (Lane 4), Daudi (Lane 5), HL-60 (Lane 6), Raji (Lane 7), MCF7 (Lane 8), Mouse Testis (Lane 9), Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Relative expression was observed in cell lines as well as in tissues.