MAB2169

antibody from Novus Biologicals

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2169

Provider

Novus Biologicals

Product name

Rat Monoclonal STAT6 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat STAT6 in Western blots.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse STAT6 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line untreated (-) or treated (+) with 25 μg Recombinant Mouse IL-4 (Catalog # 404-ML) for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse STAT6 Monoclonal Antibody (Catalog # MAB2169), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT6 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.