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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX113273 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113273, RRID:AB_10721049
- Product name
- STAT6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Cross-talk between human airway epithelial cells and 3T3-J2 feeder cells involves partial activation of human MET by murine HGF.
A high-throughput pipeline for validation of antibodies.
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Hynds RE, Gowers KHC, Nigro E, Butler CR, Bonfanti P, Giangreco A, Prêle CM, Janes SM
PloS one 2018;13(5):e0197129
PloS one 2018;13(5):e0197129
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58
Oncotarget 2016 Feb 2;7(5):6146-58
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX113273 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAT6 antibody immunoprecipitates STAT6 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 50 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 £gg of STAT6 antibody (GTX113273) 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody (GTX113273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using STAT6 antibody(GTX113273) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAT6 antibody detects STAT6 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. STAT6 antibody (GTX113273) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAT6 antibody immunoprecipitates STAT6 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ?g preimmune rabbit IgG B. 5 ?g of STAT6 antibody (GTX113273) The precipitated DNA was detected by PCR with primer set targeting to GATA3 gene locus.
- Validation comment
- ChIP
