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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-34814 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAT6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Solitary Fibrous Tumor.
- Concentration
- 0.22 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAT6 using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a STAT6 polyclonal antibody (Product # PA5-34814) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of STAT6 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: STAT6 Polyclonal Antibody (Product # PA5-34814) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using STAT6 Polyclonal Antibody (Product # PA5-34814) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAT6 Polyclonal Antibody detects STAT6 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. STAT6 Polyclonal Antibody (Product # PA5-34814) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAT6 Polyclonal Antibody immunoprecipitates STAT6 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 50 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 µg of STAT6 Polyclonal Antibody (Product # PA5-34814) 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 Polyclonal Antibody (Product # PA5-34814) diluted at 1:1,000.
