12-9013-41

antibody from Invitrogen Antibodies
Targeting: STAT6 D12S1644, IL-4-STAT
Provider product page for 12-9013-41

Antibody data

Product number
12-9013-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry. Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
CHI2S4N
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
STAT6 protein structure - 12-9013-41 shown in red.