NBP1-85345

antibody from Novus Biologicals

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85345

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85345, RRID:AB_11042070

Product name

Rabbit Polyclonal STAT6 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat STAT6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: STAT6 Antibody [NBP1-85345] - Simple Western lane view shows a specific band for STAT6 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: STAT6 Antibody [NBP1-85345] - Electropherogram image(s) of corresponding Simple Western lane view. STAT6 antibody was used at 1:20 dilution on RT-4 lysate(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STAT6 Antibody [NBP1-85345] - Analysis in human cell line Daudi.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STAT6 Antibody [NBP1-85345] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STAT6 Antibody [NBP1-85345] - Analysis in human cell lines A-549 and U-251MG using Anti-STAT6 antibody. Corresponding STAT6 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human Fallopian tube shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human skin shows weak to moderate nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human tonsil shows moderate nuclear and cytoplasmic positivity in non-germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining in human fallopian tube and skeletal muscle tissues using NBP1-85345 antibody. Corresponding STAT6 RNA-seq data are presented for the same tissues.