MA5-15659

antibody from Invitrogen Antibodies

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15659

Provider

Invitrogen Antibodies

Product name

STAT6 Monoclonal Antibody (7D3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15659 targets STAT6 in indirect ELISA, IF and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples. The MA5-15659 immunogen is purified recombinant fragment of human STAT6 expressed in E. Coli. MA5-15659 detects STAT6 which has a predicted molecular weight of approximately 94kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

7D3

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Patients with T⁺/low NK⁺ IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.

Fuchs S, Rensing-Ehl A, Erlacher M, Vraetz T, Hartjes L, Janda A, Rizzi M, Lorenz MR, Gilmour K, de Saint-Basile G, Roifman CM, Cheuk S, Gennery A, Thrasher AJ, Fuchs I, Schwarz K, Speckmann C, Ehl S
European journal of immunology 2014 Oct;44(10):3129-40

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using STAT6 monoclonal antibody (Product # MA5-15659) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of STAT6 was performed using 70% confluent log phase HeLa cells treated with 10 ng of IL4 for 30 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with STAT6 Mouse Monoclonal Antibody (Product # MA5-15659) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nucleus localization. Panel e shows untreated cells with cytoplasmic signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using STAT6 monoclonal antibody (Product # MA5-15659) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous STAT6 protein at specific gene loci using Anti-STAT6 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-STAT6 Mouse Monoclonal Antibody (Product # MA5-15659, 8 µL) on sheared chromatin from 2 million HeLa cells starved overnight then treated with IL4 (100 ng/mL for 30 min) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over DMD promoter, MS4A1 promoter (active) and SAT2 satellite repeats (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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