PA1-38675

antibody from Invitrogen Antibodies

Targeting: STAT6 D12S1644, IL-4-STAT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-38675

Provider

Invitrogen Antibodies

Product name

STAT6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine, mouse, porcine, rabbit based on sequence homology.

Concentration

0.6 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), U-87 MG (Lane 4), HT-29 (Lane 5), Caco-2 (Lane 6) and A-431 (Lane 7). The blot was probed with Anti-STAT6 Polyclonal Antibody (Product # PA5-32603, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 94 kDa band corresponding to STAT6 was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), U-87 MG (Lane 4), HT-29 (Lane 5), Caco-2 (Lane 6) and A-431 (Lane 7). The blot was probed with Anti-STAT6 Polyclonal Antibody (Product # PA5-32603, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 94 kDa band corresponding to STAT6 was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), U-87 MG (Lane 4), HT-29 (Lane 5), Caco-2 (Lane 6) and A-431 (Lane 7). The blot was probed with Anti-STAT6 Polyclonal Antibody (Product # PA5-32603, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 94 kDa band corresponding to STAT6 was observed across the cell lines tested.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of STAT6 using anti-STAT6 Polyclonal Antibody (Product # PA5-32603) in Placenta Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:150.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous STAT6 protein at specific gene loci using Anti-STAT6 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-STAT6 Rabbit Polyclonal Antibody (Product # PA5-32603, 12 µl) on sheared chromatin from 2 million HeLa cells treated with IL4 (overnight serum-starvation followed by 100 ng/mL IL4 for 30 min) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over MS4A1 and DMD promoters, DMD intron 2 (active) and SAT2 satellite repeats (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.