Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00152PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00152PU-N, RRID:AB_1007514
- Product name
- anti STAT6 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjuagted to KLH
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C12
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of Endogenous STAT6: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblots was probed with mab STAT6-8C12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431.Lane 2: SW480 Lane 3: SW620Lane 4: HT29.Lane 5: MCF7.Lane 6: MDA-MB231Lane 7: T47D