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- Antigen structure
- References [1]
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- Western blot [6]
- Flow cytometry [1]
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- Product number
- MA5-17198 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAV1 Monoclonal Antibody (2E11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17198 targets VAV1 in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17198 immunogen is purified recombinant fragment of human VAV1 (amino acids: 121-324) expressed in E. Coli. MA5-17198 detects VAV1 which has a predicted molecular weight of approximately 98.3kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Filgotinib suppresses HIV-1-driven gene transcription by inhibiting HIV-1 splicing and T cell activation.
Yeh YJ, Jenike KM, Calvi RM, Chiarella J, Hoh R, Deeks SG, Ho YC
The Journal of clinical investigation 2020 Sep 1;130(9):4969-4984
The Journal of clinical investigation 2020 Sep 1;130(9):4969-4984
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAV1 using a VAV1 monoclonal antibody (Product # MA5-17198) against a human VAV1 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAV1 using VAV1 monoclonal antibody (Product # MA5-17198) in HEK293 (1) and VAV1 (AA: 121-324) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-VAV1 Monoclonal Antibody (2E11) (Product # MA5-17198) and a 98kDa band corresponding to VAV1 was observed in Raji, MOLT-4, Jurkat, THP-1 but not in T-47D and MCF7 which are reported to be negative. Whole cell extracts (30ug) of Raji (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3), THP-1 (Lane 4), T-47D (Lane 5) and MCF7 (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAV1 using a VAV1 monoclonal antibody (Product # MA5-17198) against a human VAV1 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAV1 using VAV1 monoclonal antibody (Product # MA5-17198) in HEK293 (1) and VAV1 (AA: 121-324) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAV1 using VAV1 monoclonal antibody (Product # MA5-17198) in Jurkat (1) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using VAV1 monoclonal antibody (Product # MA5-17198) (green) and negative control (red).
