PA1-84165

antibody from Invitrogen Antibodies

Targeting: APP AD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-84165

Provider

Invitrogen Antibodies

Product name

Amyloid Precursor Protein Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-84165 recognizes both intact APP and also the C99 fragment generated by Beta-secretase. The sequence recognized by this antibody corresponds to amino acids 85-99 of the C99 fragment. Heat-mediated antigen retrieval is recommended for the staining of paraffin sections.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Depletion of microglia immediately following traumatic brain injury in the pediatric rat: Implications for cellular and behavioral pathology.

Hanlon LA, Raghupathi R, Huh JW
Experimental neurology 2019 Jun;316:39-51

---

Alzheimer's disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric amyloid-β peptide injection in rats.

Zussy C, Brureau A, Keller E, Marchal S, Blayo C, Delair B, Ixart G, Maurice T, Givalois L
PloS one 2013;8(1):e53117

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates with a Amyloid Precursor Protein polyclonal antibody (Product # PA1-84165)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Amyloid Precursor Protein Polyclonal Antibody (Product # PA1-84165) and a 120, 90kDa band corresponding to Amyloid Precursor Protein was observed across the panel tested except for K-562, HEL 92.1.7, Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported to be negative. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), K-562 (Lane 2), HEL 92.1.7 (Lane 3), Mouse Brain (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Brain (Lane 6), Rat Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of rat brain using a Amyloid Precursor Protein polyclonal antibody (Product # PA1-84165)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 12 APP processing. Effects of oAbeta 25-35 (10 ug/rat) icv injection on APP processing in the frontal cortex, amygdala, hippocampus and hypothalamus, determined by western blot in untreated control rats and 6 weeks after Abeta 25-35 scrambled amyloid peptide (10 ug/rat; negative control) or oAbeta 25-35 injection. APP (125 KDa) and C99 (13 KDa) variations were normalized with beta-tubulin (beta-tub, 55 KDa) variations and compared with non-injected rats (control group: C). The results are expressed as means +- SEM. *p