ABIN350071

antibody from antibodies-online

Targeting: APP AD1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN350071

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN350071, RRID:AB_10782160

Product name

anti-Amyloid beta (A4) Precursor Protein (APP) (AA 400-450) antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide from aa region 400-450 of human APP conjugated to an immunogenic carrier protein was used as the antigen.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

AA 400-450

Isotype

IgG

Vial size

500 μg

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Handling

Avoid freeze and thaw cycles.

References

Submitted references

Distinct intramembrane cleavage of the beta-amyloid precursor protein family resembling gamma-secretase-like cleavage of Notch.

Gu Y, Misonou H, Sato T, Dohmae N, Takio K, Ihara Y
The Journal of biological chemistry 2001 Sep 21;276(38):35235-8

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Appican, the proteoglycan form of the amyloid precursor protein, contains chondroitin sulfate E in the repeating disaccharide region and 4-O-sulfated galactose in the linkage region.

Tsuchida K, Shioi J, Yamada S, Boghosian G, Wu A, Cai H, Sugahara K, Robakis NK
The Journal of biological chemistry 2001 Oct 5;276(40):37155-60

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Expression of the APP gene family in brain cells, brain development and aging.

Sandbrink R, Mönning U, Masters CL, Beyreuther K
Gerontology 1997;43(1-2):119-31

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APP gene family. Alternative splicing generates functionally related isoforms.

Sandbrink R, Masters CL, Beyreuther K
Annals of the New York Academy of Sciences 1996 Jan 17;777:281-7

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The Alzheimer amyloid precursor proteoglycan (appican) is present in brain and is produced by astrocytes but not by neurons in primary neural cultures.

Shioi J, Pangalos MN, Ripellino JA, Vassilacopoulou D, Mytilineou C, Margolis RU, Robakis NK
The Journal of biological chemistry 1995 May 19;270(20):11839-44

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Purification and tissue level of the beta-amyloid peptide precursor of rat brain.

Potempska A, Styles J, Mehta P, Kim KS, Miller DL
The Journal of biological chemistry 1991 May 5;266(13):8464-9

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The sequence of the two extra exons in rat preA4.

Kang J, Müller-Hill B
Nucleic acids research 1989 Mar 11;17(5):2130

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Alzheimer's disease amyloidogenic glycoprotein: expression pattern in rat brain suggests a role in cell contact.

Shivers BD, Hilbich C, Multhaup G, Salbaum M, Beyreuther K, Seeburg PH
The EMBO journal 1988 May;7(5):1365-70

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Amyloid beta protein precursor is possibly a heparan sulfate proteoglycan core protein.

Schubert D, Schroeder R, LaCorbiere M, Saitoh T, Cole G
Science (New York, N.Y.) 1988 Jul 8;241(4862):223-6