700254

antibody from Invitrogen Antibodies

Targeting: APP AD1

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

700254

Provider

Invitrogen Antibodies

Product name

beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Cross reactivity to Abeta [1-40] is not observed in sandwich ELISA. In addition, in antigen ELISA cross-reactivity is not observed with Abeta [1-37], Abeta [1-38], Abeta [1-40], or Abeta [1-43] when used at low antibody concentrations (up to 30 ng/mL).

Antibody clone number

H31L21

Concentration

0.5 mg/mL

References

Submitted references

Oral nimodipine treatment has no effect on amyloid pathology or neuritic dystrophy in the 5XFAD mouse model of amyloidosis.

Sadleir KR, Popovic J, Khatri A, Vassar R
PloS one 2022;17(2):e0263332

---

Farnesyltransferase inhibitor LNK-754 attenuates axonal dystrophy and reduces amyloid pathology in mice.

Cuddy LK, Alia AO, Salvo MA, Chandra S, Grammatopoulos TN, Justman CJ, Lansbury PT Jr, Mazzulli JR, Vassar R
Molecular neurodegeneration 2022 Aug 20;17(1):54

---

Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer's Disease.

Hinteregger B, Loeffler T, Flunkert S, Neddens J, Bayer TA, Madl T, Hutter-Paier B
Journal of Alzheimer's disease : JAD 2021;80(3):1151-1168

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Alzheimer's disease BIN1 coding variants increase intracellular Aβ levels by interfering with BACE1 recycling.

Perdigão C, Barata MA, Burrinha T, Guimas Almeida C
The Journal of biological chemistry 2021 Sep;297(3):101056

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Neuronal spreading and plaque induction of intracellular Aβ and its disruption of Aβ homeostasis.

Roos TT, Garcia MG, Martinsson I, Mabrouk R, Israelsson B, Deierborg T, Kobro-Flatmoen A, Tanila H, Gouras GK
Acta neuropathologica 2021 Oct;142(4):669-687

---

Identification of key transcriptome biomarkers based on a vital gene module associated with pathological changes in Alzheimer's disease.

Zhang T, Shen Y, Guo Y, Yao J
Aging 2021 May 24;13(11):14940-14967

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Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype: Involvement of proteasome activation.

Mladenovic Djordjevic AN, Kapetanou M, Loncarevic-Vasiljkovic N, Todorovic S, Athanasopoulou S, Jovic M, Prvulovic M, Taoufik E, Matsas R, Kanazir S, Gonos ES
Free radical biology & medicine 2021 Jan;162:88-103

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Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer's and Creutzfeldt-Jakob Disease: A Micromorphological Pilot Study on 20 Brains.

Jankovska N, Olejar T, Matej R
International journal of molecular sciences 2021 Feb 20;22(4)

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Microglial response to experimental periodontitis in a murine model of Alzheimer's disease.

Kantarci A, Tognoni CM, Yaghmoor W, Marghalani A, Stephens D, Ahn JY, Carreras I, Dedeoglu A
Scientific reports 2020 Oct 29;10(1):18561

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Comparison of regional flortaucipir PET with quantitative tau immunohistochemistry in three subjects with Alzheimer's disease pathology: a clinicopathological study.

Pontecorvo MJ, Keene CD, Beach TG, Montine TJ, Arora AK, Devous MD Sr, Navitsky M, Kennedy I, Joshi AD, Lu M, Serrano GE, Sue LI, Intorcia AJ, Rose SE, Wilson A, Hellstern L, Coleman N, Flitter M, Aldea P, Fleisher AS, Mintun MA, Siderowf A
EJNMMI research 2020 Jun 15;10(1):65

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Synergistic toxicity between tau and amyloid drives neuronal dysfunction and neurodegeneration in transgenic C. elegans.

Benbow SJ, Strovas TJ, Darvas M, Saxton A, Kraemer BC
Human molecular genetics 2020 Feb 1;29(3):495-505

---

Dual dose-dependent effects of fingolimod in a mouse model of Alzheimer's disease.

Carreras I, Aytan N, Choi JK, Tognoni CM, Kowall NW, Jenkins BG, Dedeoglu A
Scientific reports 2019 Jul 29;9(1):10972

---

TREM2 function impedes tau seeding in neuritic plaques.

Leyns CEG, Gratuze M, Narasimhan S, Jain N, Koscal LJ, Jiang H, Manis M, Colonna M, Lee VMY, Ulrich JD, Holtzman DM
Nature neuroscience 2019 Aug;22(8):1217-1222

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Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice.

Ilievski V, Zuchowska PK, Green SJ, Toth PT, Ragozzino ME, Le K, Aljewari HW, O'Brien-Simpson NM, Reynolds EC, Watanabe K
PloS one 2018;13(10):e0204941

---

Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.

Milanovic D, Petrovic S, Brkic M, Avramovic V, Perovic M, Ivkovic S, Glibetic M, Kanazir S
Nutrients 2018 Sep 6;10(9)

---

ER stress is not elevated in the 5XFAD mouse model of Alzheimer's disease.

Sadleir KR, Popovic J, Vassar R
The Journal of biological chemistry 2018 Nov 30;293(48):18434-18443

---

Alzheimer's Disease-Associated β-Amyloid Is Rapidly Seeded by Herpesviridae to Protect against Brain Infection.

Eimer WA, Vijaya Kumar DK, Navalpur Shanmugam NK, Rodriguez AS, Mitchell T, Washicosky KJ, György B, Breakefield XO, Tanzi RE, Moir RD
Neuron 2018 Jul 11;99(1):56-63.e3

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Heterogeneous Association of Alzheimer's Disease-Linked Amyloid-β and Amyloid-β Protein Precursor with Synapses.

Willén K, Sroka A, Takahashi RH, Gouras GK
Journal of Alzheimer's disease : JAD 2017;60(2):511-524

---

HIV-1 counteracts an innate restriction by amyloid precursor protein resulting in neurodegeneration.

Chai Q, Jovasevic V, Malikov V, Sabo Y, Morham S, Walsh D, Naghavi MH
Nature communications 2017 Nov 15;8(1):1522

---

Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP.

Klementieva O, Willén K, Martinsson I, Israelsson B, Engdahl A, Cladera J, Uvdal P, Gouras GK
Nature communications 2017 Mar 13;8:14726

---

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

Ubelmann F, Burrinha T, Salavessa L, Gomes R, Ferreira C, Moreno N, Guimas Almeida C
EMBO reports 2017 Jan;18(1):102-122

---

Identification of natural products with neuronal and metabolic benefits through autophagy induction.

Fan Y, Wang N, Rocchi A, Zhang W, Vassar R, Zhou Y, He C
Autophagy 2017 Jan 2;13(1):41-56

---

A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease.

Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, Huang S, Levine B, Vassar R, He C
PLoS genetics 2017 Aug;13(8):e1006962

---

Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A.

Willén K, Edgar JR, Hasegawa T, Tanaka N, Futter CE, Gouras GK
Molecular neurodegeneration 2017 Aug 23;12(1):61

---

Active duty service members who sustain a traumatic brain injury have chronically elevated peripheral concentrations of Aβ40 and lower ratios of Aβ42/40.

Lejbman N, Olivera A, Heinzelmann M, Feng R, Yun S, Kim HS, Gill J
Brain injury 2016;30(12):1436-1441

---

The fornix provides multiple biomarkers to characterize circuit disruption in a mouse model of Alzheimer's disease.

Badea A, Kane L, Anderson RJ, Qi Y, Foster M, Cofer GP, Medvitz N, Buckley AF, Badea AK, Wetsel WC, Colton CA
NeuroImage 2016 Nov 15;142:498-511

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Early Alzheimer's disease-type pathology in the frontal cortex of wild mountain gorillas (Gorilla beringei beringei).

Perez SE, Sherwood CC, Cranfield MR, Erwin JM, Mudakikwa A, Hof PR, Mufson EJ
Neurobiology of aging 2016 Mar;39:195-201

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Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer's disease.

Sadleir KR, Kandalepas PC, Buggia-Prévot V, Nicholson DA, Thinakaran G, Vassar R
Acta neuropathologica 2016 Aug;132(2):235-256

---

Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse.

Dinkins MB, Enasko J, Hernandez C, Wang G, Kong J, Helwa I, Liu Y, Terry AV Jr, Bieberich E
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 17;36(33):8653-67

---

Aβ reduction in BACE1 heterozygous null 5XFAD mice is associated with transgenic APP level.

Sadleir KR, Eimer WA, Cole SL, Vassar R
Molecular neurodegeneration 2015 Jan 7;10:1

---

Microglia constitute a barrier that prevents neurotoxic protofibrillar Aβ42 hotspots around plaques.

Condello C, Yuan P, Schain A, Grutzendler J
Nature communications 2015 Jan 29;6:6176

---

Cerebral cortical Aβ42 and PHF-τ in 325 consecutive brain autopsies stratified by diagnosis, location, and APOE.

Postupna N, Keene CD, Crane PK, Gonzalez-Cuyar LF, Sonnen JA, Hewitt J, Rice S, Howard K, Montine KS, Larson EB, Montine TJ
Journal of neuropathology and experimental neurology 2015 Feb;74(2):100-9

---

Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.

Wu Z, Guo Z, Gearing M, Chen G
Nature communications 2014 Jun 13;5:4159

---

Experimental traumatic brain injury induces rapid aggregation and oligomerization of amyloid-beta in an Alzheimer's disease mouse model.

Washington PM, Morffy N, Parsadanian M, Zapple DN, Burns MP
Journal of neurotrauma 2014 Jan 1;31(1):125-34

---

Exosome reduction in vivo is associated with lower amyloid plaque load in the 5XFAD mouse model of Alzheimer's disease.

Dinkins MB, Dasgupta S, Wang G, Zhu G, Bieberich E
Neurobiology of aging 2014 Aug;35(8):1792-800

---

Elevated Aβ42 in aged, non-demented individuals with cerebral atherosclerosis.

Sadleir KR, Bennett DA, Schneider JA, Vassar R
Current Alzheimer research 2013 Oct;10(8):785-9

---

Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice.

Tóth ME, Szegedi V, Varga E, Juhász G, Horváth J, Borbély E, Csibrány B, Alföldi R, Lénárt N, Penke B, Sántha M
Cell stress & chaperones 2013 Nov;18(6):759-71

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Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause.

Zhang QG, Wang RM, Scott E, Han D, Dong Y, Tu JY, Yang F, Reddy Sareddy G, Vadlamudi RK, Brann DW
Brain : a journal of neurology 2013 May;136(Pt 5):1432-45

---

Neuron loss in the 5XFAD mouse model of Alzheimer's disease correlates with intraneuronal Aβ42 accumulation and Caspase-3 activation.

Eimer WA, Vassar R
Molecular neurodegeneration 2013 Jan 14;8:2

---

Alzheimer's disease pathology in the neocortex and hippocampus of the western lowland gorilla (Gorilla gorilla gorilla).

Perez SE, Raghanti MA, Hof PR, Kramer L, Ikonomovic MD, Lacor PN, Erwin JM, Sherwood CC, Mufson EJ
The Journal of comparative neurology 2013 Dec 15;521(18):4318-38

---

Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide.

Li W, Tang Y, Fan Z, Meng Y, Yang G, Luo J, Ke ZJ
Molecular neurodegeneration 2013 Aug 10;8:27

---

Hypoxia due to cardiac arrest induces a time-dependent increase in serum amyloid β levels in humans.

Zetterberg H, Mörtberg E, Song L, Chang L, Provuncher GK, Patel PP, Ferrell E, Fournier DR, Kan CW, Campbell TG, Meyer R, Rivnak AJ, Pink BA, Minnehan KA, Piech T, Rissin DM, Duffy DC, Rubertsson S, Wilson DH, Blennow K
PloS one 2011;6(12):e28263

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of beta-Amyloid in formalin-fixed, paraffin-embedded human brain (left) and transgenic mouse tissue that expresses FAD mutant APP and PS1 (right) using a beta-Amyloid monoclonal antibody (Product # 700254) at a dilution of 1 µg/mL. Results show strong cytoplasmic staining in amyloid plaque.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL