Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-0100Z - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Amyloid Monoclonal Antibody (AMY-33)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This beta Amyloid Monoclonal Antibody (AMY-33) is suitable for immunohistochemical staining of alcohol-fixed paraffin-embedded or frozen tissue sections. It is necessary to pretreat the tissue with trypsin digestion.
- Antibody clone number
- AMY-33
- Concentration
- 0.5 mg/mL
Submitted references Novel Molecular Insights into Classical and Alternative Activation States of Microglia as Revealed by Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Proteomics.
Inhibiting protein-amyloid interactions with small molecules: a surface chemistry approach.
Intracerebroventricular passive immunization in transgenic mouse models of Alzheimer's disease.
Caspase-3-mediated cleavage of amyloid precursor protein and formation of amyloid Beta peptide in traumatic axonal injury.
Monoclonal antibodies inhibit in vitro fibrillar aggregation of the Alzheimer beta-amyloid peptide.
Bell-Temin H, Culver-Cochran AE, Chaput D, Carlson CM, Kuehl M, Burkhardt BR, Bickford PC, Liu B, Stevens SM Jr
Molecular & cellular proteomics : MCP 2015 Dec;14(12):3173-84
Molecular & cellular proteomics : MCP 2015 Dec;14(12):3173-84
Inhibiting protein-amyloid interactions with small molecules: a surface chemistry approach.
Inbar P, Yang J
Bioorganic & medicinal chemistry letters 2006 Feb 15;16(4):1076-9
Bioorganic & medicinal chemistry letters 2006 Feb 15;16(4):1076-9
Intracerebroventricular passive immunization in transgenic mouse models of Alzheimer's disease.
Chauhan NB, Siegel GJ
Expert review of vaccines 2004 Dec;3(6):717-25
Expert review of vaccines 2004 Dec;3(6):717-25
Caspase-3-mediated cleavage of amyloid precursor protein and formation of amyloid Beta peptide in traumatic axonal injury.
Stone JR, Okonkwo DO, Singleton RH, Mutlu LK, Helm GA, Povlishock JT
Journal of neurotrauma 2002 May;19(5):601-14
Journal of neurotrauma 2002 May;19(5):601-14
Monoclonal antibodies inhibit in vitro fibrillar aggregation of the Alzheimer beta-amyloid peptide.
Solomon B, Koppel R, Hanan E, Katzav T
Proceedings of the National Academy of Sciences of the United States of America 1996 Jan 9;93(1):452-5
Proceedings of the National Academy of Sciences of the United States of America 1996 Jan 9;93(1):452-5
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Human samples (30 µg) of Alzheimer's Disease patient CSF sample (Lane 1) and corresponding normal CSF sample (Lane 2). The blots were probed with Anti-beta-Amyloid Mouse Monoclonal Antibody (Product # 13-0100Z, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 110 kDa band corresponding to beta-Amyloid was observed in Alzheimer's Disease patient CSF sample and a 35 kDa band was observed across both human samples. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
