835104

antibody from BioLegend

Targeting: APP AD1

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

835104

Provider

BioLegend

Proper citation

BioLegend Cat#835104, RRID:AB_2632642

Product name

Purified anti-β-Amyloid, 1-11

Antibody type

Monoclonal

Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APPs) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse species of Aβ with differential propensities to fibrillize and deposit in Senile Plaques.According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD). The 770 amino acid form (APP770) has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

NAB 228

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

ELISA

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

IHC staining of anti-β-Amyloid, 1-11 antibody (clone NAB 228) on formalin-fixed paraffin-embedded human Alzheimer’s diseased neocortical brain tissue. After antigen retrieval using 70% Formic Acid for 20 minutes, the tissue was incubated with the primary antibody at 10 µg/ml for 24 hours at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.

Conjugate

Unconjugated