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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00000351-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000351-M01, RRID:AB_425312
- Product name
- APP monoclonal antibody (M01), clone 2C12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant APP.
- Antigen sequence
EVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWD
SDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEA
NQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFV
SDALLVPDKCKFLHQERMDVCETHLHWHTVAKETC
SEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEES
DNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEV
AEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYE
EATERTTSIATTTTTTTESVEEVVREKWYKEVHSG
QARWLML- Isotype
- IgG
- Antibody clone number
- 2C12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- APP monoclonal antibody (M01), clone 2C12. Western Blot analysis of APP expression in rat brain.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between TGFB1 and APP. HeLa cells were stained with anti-TGFB1 rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
