PA1-37077

antibody from Invitrogen Antibodies

Targeting: APP AD1

Immunohistochemistry

Antibody data

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Validation data

Product number: PA1-37077 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-beta Amyloid Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is Alzheimer's brain tissue.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 1 mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

APP protein structure - PA1-37077 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-beta Amyloid Polyclonal Antibody(Product # PA1-37077) and a 120, 90kDa band corresponding to beta Amyloid was observed across in PC-3 as compared to K-562, HEL 92.1.7 which are reported to be negative. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), K-562 (Lane 2) and HEL 92.1.7 (Lane 3), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-beta Amyloid Polyclonal Antibody(Product # PA5-32263) and a 120, 90kDa band corresponding to beta Amyloid was observed across in PC-3 and U-87 MG as compared to K-562, HEL 92.1.7 which are reported to be negative. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), U-87 MG (Lane 2), K-562 (Lane 3) and HEL 92.1.7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).