- PA5-19923 - Invitrogen Antibodies APP antibody

Submitted references Ethanol extract of Magnolia officinalis prevents lipopolysaccharide-induced memory deficiency via its antineuroinflammatory and antiamyloidogenic effects.

Lee YJ, Choi DY, Yun YP, Han SB, Kim HM, Lee K, Choi SH, Yang MP, Jeon HS, Jeong JH, Oh KW, Hong JT
Phytotherapy research : PTR 2013 Mar;27(3):438-47

Epigallocatechin-3-gallate prevents systemic inflammation-induced memory deficiency and amyloidogenesis via its anti-neuroinflammatory properties.

Lee YJ, Choi DY, Yun YP, Han SB, Oh KW, Hong JT
The Journal of nutritional biochemistry 2013 Jan;24(1):298-310

4-O-methylhonokiol prevents memory impairment in the Tg2576 transgenic mice model of Alzheimer's disease via regulation of β-secretase activity.

Lee YJ, Choi DY, Lee YK, Lee YM, Han SB, Kim YH, Kim KH, Nam SY, Lee BJ, Kang JK, Yun YW, Oh KW, Hong JT
Journal of Alzheimer's disease : JAD 2012;29(3):677-90

Inhibitory effect of 4-O-methylhonokiol on lipopolysaccharide-induced neuroinflammation, amyloidogenesis and memory impairment via inhibition of nuclear factor-kappaB in vitro and in vivo models.

Lee YJ, Choi DY, Choi IS, Kim KH, Kim YH, Kim HM, Lee K, Cho WG, Jung JK, Han SB, Han JY, Nam SY, Yun YW, Jeong JH, Oh KW, Hong JT
Journal of neuroinflammation 2012 Feb 19;9:35

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of rat brain cells using a APP polyclonal antibody (Product # PA5-19923) at a 20 µg/mL dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Effect of 4- O -methylhonokiol on expression of amyloidogenic proteins . (A) Immunoreactive cells of anti-BACE1 antibody were investigated in the cortex and hippocampus. (B) The present figure is representative for three different experiments with different animal brains. (C) The expression of APP, C99 and BACE1 were detected by western blotting using specific antibodies in mice brain. beta-Actin protein was used as an internal control. Each blot is representative for three experiments. The values in the western blot band indicate average density over beta-actin from 5 animals. #, Significantly different from control group ( p < 0.05). *, Significantly different from LPS-treated group ( p < 0.05). Control, saline-treated group. LPS, lipopolysaccharide. MH, 4- O -methylhonokiol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9 Effect of 4- O -methylhonokiol on LPS-induced iNOS, COX-2, APP, C99, Abeta, BACE generation, NF-kappaB activity, beta- and gamma-secretase activity in cultured astrocytes and in microglial BV-2 cells . Astrocytes and microglial BV-2 cells were treated with LPS (1 muM) and 4- O -methylhonokiol (0.5-2 muM). (A) NF-kappaB activity in astrocytes was determined by EMSA as described in Methods. (B) For supershift assays, nuclear extracts from cultured astrocytes treated with LPS (1 mug/ml) were incubated for 1 h before EMSA with specific antibodies against the p50 and p65 NF-kappaB isoforms. (C) Phosphorylation of IkappaB, and p50 and p65 translocation in the astrocytes. (D) Immunoblots of lysates from astrocytes were probed with iNOS, COX-2, APP, C99, Abeta and BACE antibodies, respectively. (E) The amounts of Abeta 1-42 were assessed by using a specific Abeta 1-42 ELISA kit as described in the Methods. (F) and (G) beta- and gamma-secretase activity in the astrocytes were determined as described in Methods. (H) Immunoblots of lysates from microglial BV-2 cells were probed with iNOS, COX-2, APP, C99 and BACE antibodies, respectively. (I) The amounts of Abeta 1-42 were assessed by using a specific Abeta 1-42 ELISA kit as described in the Methods. Values represent means +- S.D. of three independent experiments performed in triplicate. The values in the western blot band indicate average density over beta-actin from three animals. #, Significantly different from control group

PA5-19923

Antibody data