LS-C99554

antibody from LSBio

Targeting: USP14 TGT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C99554

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C99554, RRID:AB_2241530

Product name

USP14 Antibody (aa1-30) LS-C99554

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

The anti-USP14 antibody is used in Western blot to detect USP14 in USP14-transfected HeLa cell lysates. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of anti-USP14 antibody in A2058 cell line lysates (35 ug/lane). USP14 (arrow) was detected using the purified antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of USP14 (arrow) using rabbit polyclonal USP14 Antibody (K7). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP14 gene.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.