14517-1-AP

antibody from Proteintech Group

Targeting: USP14 TGT

Provider product page for 14517-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [41]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 14517-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14517-1-AP, RRID:AB_2257124
Product name: USP14 antibody
Antibody type: Polyclonal
Description: KD/KO validated USP14 antibody (Cat. #14517-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

USP14 protein structure - 14517-1-AP shown in red.

Submitted references Regulation of sepsis-associated acute kidney injury by ELAVL1 through USP14/NCOA4-mediated ferroptosis in renal tubular epithelial cells.

Zhu X, Zheng P, Jin H, Zhao Q, Kong K, Zheng L, Huang X, Zheng M
Molecular and cellular biochemistry 2026 May;481(5):2253-2266

MGMT upregulation mediates Temozolomide resistance conferred USP5 dependency.

Bhardwaj S, Sanjay, Sharma D, Taneja L, Yadav BK, Yadav AK
Scientific reports 2026 Jan 24;16(1):6118

Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination.

Yunxin L, Xirui X, Xinyi Z, Chenjing W, Ting Z, Kanghui F, Haixia Y, Zude C, Wan H, Yubing Z, Xianjun F
Journal of translational medicine 2026 Feb 26;24(1)

USP14 promotes epithelial-mesenchymal transition and functions as a multifaceted biomarker in lung adenocarcinoma.

Wu C, Chen Z, Yang J, Shi H, Xing Y, Liu H, Shen Z, Xu C, Ding C, Ding H, Zhao J
Discover oncology 2026 Feb 21;17(1)

HAO2 inhibits the growth and lipid metabolism of head and neck squamous cell carcinoma by enhancing MYH9 ubiquitination mediated by STUB1 and USP14.

Yan D, Tong W, You W, Zhou W, Huang S, Liu H, Huang X, Luo Q, Ye J
Biochimica et biophysica acta. Molecular basis of disease 2026 Apr 7;1872(6):168239

Association Between Proteasome 26S Subunit, Non-ATPase 3 Methylation and Insulin β Cell Apoptosis in Type 2 Diabetic Mellitus.

Huang G, He G, Chen S, Mai L
Diabetes, metabolic syndrome and obesity : targets and therapy 2025;18:3203-3214

USP14-mediated stabilization of ACTN1 maintains mesenchymal characteristics in glioblastoma.

Qiu W, Shi X, Wei R, Song J, Ling Y, Hu Y, Song S, Chen Y, Yang H, Luo P, Chu L
Communications biology 2025 Nov 25;8(1):1680

HSPA9 contributes to tumor progression and ferroptosis resistance by enhancing USP14-driven SLC7A11 deubiquitination in multiple myeloma.

Shen N, Xia Y, Shen X, Hua W, Shi M, Chen L
Cell reports 2025 May 27;44(5):115720

NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma.

Gong B, Liu Y, Yan W, Cheng C, Yang H, Huang J, Liu Q, Liu Y, Guo J, Deng X, Zhou B, Zheng D, Liu X, Liu Z, Fang W
Molecular cancer 2025 May 26;24(1):152

USP14 inhibits mitophagy and promotes tumorigenesis and chemosensitivity through deubiquitinating BAG4 in microsatellite instability-high colorectal cancer.

Wang Z, Yu C, Xie G, Tao K, Yin Z, Lv Q
Molecular medicine (Cambridge, Mass.) 2025 May 2;31(1):163

Mettl1 knockdown alleviates cardiac I/R injury in mice by inactivating the Mettl1-CYLD-P53 positive feedback loop.

Yu ST, Sun ZY, Li N, Qu ZZ, Wang CH, Ju TT, Liu YQ, Mei ZT, Liu KW, Lu MX, Huang M, Li Y, Dou SK, Jiang JH, Zhang YZ, Huang CH, Pang XC, Jia YQ, Dong XH, Wu F, Zhang Y, Li WH, Yang BF, Du WJ
Acta pharmacologica Sinica 2025 Mar;46(3):592-605

Piceatannol upregulates USP14-mediated GPX4 deubiquitination to inhibit neuronal ferroptosis caused by cerebral ischemia-reperfusion in mice.

Zhao Q, Wang X, Zhang T, Guo S, Liu X, Wan S, Guo Y, Zhang Q, Li J, Wang S, Dong L
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2025 Mar;197:115281

Lupeol induces apoptosis of glioma cells via governing ubiquitination-mediated SPARC turnover.

Wei Y, Wan Y, Duan S, Hu Q, Xu N
Tissue & cell 2025 Dec;97:103039

Identification of AKOS, a Chikungunya virus inhibitor, as a USP14 inhibitor for colorectal cancer treatment.

Lu B, Sun YY, Zhou JH, Chen DN, Guo Y, Chen YL, Pan CH, Chen ZY, He QJ, Yuan M, Cao J, Jiang L, Yang B
Acta pharmacologica Sinica 2025 Dec;46(12):3302-3313

Ubiquitin-specific protease 14 inhibition promotes mitophagy and attenuates neural apoptosis after spinal cord injury.

Wu D, Chen D, Chen H, Yu Z, Feng H, Zhou L, Chen Z, Shi T, Wu R, Zhang Y, Sun L, Wang Z, Liu W
Scientific reports 2025 Dec 9;15(1):43741

Ultrasmall Nanoparticles Mitigate Tau Hyperphosphorylation to Restore Synaptic Integrity and Boost Cognitive Function in Alzheimer's Disease.

Xu H, Wang G, Jiang Z, Han Y, Zhao W, Zhang H, Liu H, Liu H, Li Z, Ji F
Advanced healthcare materials 2025 Aug;14(20):e2500941

miR-30a enhanced RIG-I-mediated type I interferon antiviral response by targeting USP14.

Zhang J, Wang Y, Sun N, Zou B, Wang Z, Yin H, Xie J, Xia B, Sun N
Microbiology spectrum 2025 Aug 5;13(8):e0018825

The cohesin-associated protein Pds5A governs the meiotic spindle assembly via deubiquitination of Kif5B in oocytes.

Zhang Y, Bai J, Xiong B
Science advances 2025 Apr 11;11(15):eadt6159

Synergistic Effect of Ubiquitin-Specific Protease 14 and Poly(ADP-Ribose) Glycohydrolase Co-Inhibition in BRCA1-Mutant, Poly(ADP-Ribose) Polymerase Inhibitor-Resistant Triple-Negative Breast Cancer Cells.

Li P, Zhu X, Qu H, Han Z, Yao X, Wei Y, Li B, Chen H
OncoTargets and therapy 2024;17:741-753

USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination.

Liu C, Zhou S, Tang W
Oral diseases 2024 Oct;30(7):4255-4265

Interplay between acetylation and ubiquitination controls PSAT1 protein stability in lung adenocarcinoma.

Liu Y, Xun W, Zhao T, Huang M, Sun L, Wen G, Kang X, Wang J, Han T
Communications biology 2024 Oct 21;7(1):1365

USP14 modulates cell pyroptosis and ameliorates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing SIRT3.

Zhang Z, Jin B, Zhang Y, Yang M, Wang C, Zhu Y, Li T, Lin J, Yang M, Cheng Y, Xu S, He K, Xu J, Mi Y, Jiang J, Sun Z
Free radical biology & medicine 2024 Nov 20;225:741-757

USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase.

Chen Y, Feng X, Wu Z, Yang Y, Rao X, Meng R, Zhang S, Dong X, Xu S, Wu G, Jie X
Journal of biomedical science 2024 May 28;31(1):55

GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4.

Chen Y, Feng Y, Lin Y, Zhou X, Wang L, Zhou Y, Lin K, Cai L
British journal of cancer 2024 Mar;130(5):755-768

C-MYC-activated lncRNA SNHG20 accelerates the proliferation of diffuse large B cell lymphoma via USP14-mediated deubiquitination of β-catenin.

Wang C, Fu W, Zhang Y, Hu X, Xu Q, Tong X
Biology direct 2024 Jun 18;19(1):47

Triptolide attenuates cardiac remodeling by inhibiting pyroptosis and EndMT via modulating USP14/Keap1/Nrf2 pathway.

Ba L, E M, Wang R, Wu N, Wang R, Liu R, Feng X, Qi H, Sun H, Qiao G
Heliyon 2024 Jan 30;10(2):e24010

PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4.

Tao H, Song SJ, Fan ZW, Li WT, Jin X, Jiang W, Bai J, Shi ZZ
Antioxidants (Basel, Switzerland) 2024 Jan 17;13(1)

USP14 promotes the proliferation of cervical cancer via upregulating β-catenin.

Song H, Qiu J, Hua K
Environmental toxicology 2024 Feb;39(2):1031-1043

USP14 Positively Modulates Head and Neck Squamous Carcinoma Tumorigenesis and Potentiates Heat Shock Pathway through HSF1 Stabilization.

Wang J, Xiang Y, Xie Z, Fan M, Fang S, Wan H, Zhao R, Zeng F, Hua Q
Cancers 2023 Sep 1;15(17)

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway.

Hunter JE, Campbell AE, Hannaway NL, Kerridge S, Luli S, Butterworth JA, Sellier H, Mukherjee R, Dhillon N, Sudhindar PD, Shukla R, Brownridge PJ, Bell HL, Coxhead J, Taylor L, Leary P, Hasoon MSR, Collins I, Garrett MD, Eyers CE, Perkins ND
The Biochemical journal 2022 Oct 14;479(19):2063-2086

Derepression of the USP22-FASN axis by p53 loss under oxidative stress drives lipogenesis and tumorigenesis.

Han Z, Liu M, Xie Y, Zeng K, Zhan Z, Chen Y, Wang L, Chen X, Luo Y, Zeng Y, Zhan H, Lin Y, Zhang K, Zhu X, Liu S, Luo X, Zhou A
Cell death discovery 2022 Nov 4;8(1):445

Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.

Ming SL, Zhang S, Wang Q, Zeng L, Zhou LY, Wang MD, Ma YX, Han LQ, Zhong K, Zhu HS, Bai YL, Yang GY, Wang J, Chu BB
Autophagy 2022 Aug;18(8):1801-1821

SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis.

Zhu T, Wang Z, Zou T, Xu L, Zhang S, Chen Y, Chen C, Zhang W, Wang S, Ding Q, Xu G
Frontiers in pharmacology 2021;12:769647

The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities.

Fang G, Fu Y, Li S, Qiu J, Kuang M, Lin S, Li C, Ding Y
The Journal of biological chemistry 2020 May 15;295(20):7018-7032

SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.

Xu J, Deng Y, Wang Y, Sun X, Chen S, Fu G
Cell proliferation 2020 Feb;53(2):e12738

CyclinB1 deubiquitination by USP14 regulates cell cycle progression in breast cancer.

Liu B, Liu Y, Wang Y, Xie C, Gan M, Han T, Cao J, Wang J
Pathology, research and practice 2019 Oct;215(10):152592

BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin.

Cheng Z, Xin H, Han T
Cell adhesion & migration 2019 Dec;13(1):249-259

Proteasome-associated cysteine deubiquitinases are molecular targets of environmental optical brightener compounds.

Castro I, Ekinci E, Huang X, Cheaito HA, Ahn YH, Olivero-Verbel J, Dou QP
Journal of cellular biochemistry 2019 Aug;120(8):14065-14075

USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination.

Sharma A, Alswillah T, Singh K, Chatterjee P, Willard B, Venere M, Summers MK, Almasan A
Autophagy 2018;14(11):1976-1990

USP14 as a novel prognostic marker promotes cisplatin resistance via Akt/ERK signaling pathways in gastric cancer.

Fu Y, Ma G, Liu G, Li B, Li H, Hao X, Liu L
Cancer medicine 2018 Nov;7(11):5577-5588

Overexpression of ubiquitin specific peptidase 14 predicts unfavorable prognosis in esophageal squamous cell carcinoma.

Zhang B, Li M, Huang P, Guan XY, Zhu YH
Thoracic cancer 2017 Jul;8(4):344-349

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP(USP14 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line