Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB7658 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NAALADase-2/NAALAD2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NAALADase-2/NAALAD2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NAALADase-2 is observed and no cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like 2, or rhNAALADase-like 1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human NAALADase-2/NAALAD2 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- NAALADase-2/NAALAD2 in Human Testis. NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/ NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.