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- Antibody Data
- Antigen structure
- References [7]
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- Western blot [1]
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- Product number
- 200-401-194 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-194, RRID:AB_2253725
- Product name
- Anti-RFX5 (aa 320 to 494) (RABBIT) Antibody - 200-401-194
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Therapeutic targeting of EP300/CBP by bromodomain inhibition in hematologic malignancies.
RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A.
Forkhead transcription factor FOXO3a mediates interferon-γ-induced MHC II transcription in macrophages.
The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma.
In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport.
Interferon gamma repression of collagen (COL1A2) transcription is mediated by the RFX5 complex.
The RFX family interacts at the collagen (COL1A2) start site and represses transcription.
Nicosia L, Spencer GJ, Brooks N, Amaral FMR, Basma NJ, Chadwick JA, Revell B, Wingelhofer B, Maiques-Diaz A, Sinclair O, Camera F, Ciceri F, Wiseman DH, Pegg N, West W, Knurowski T, Frese K, Clegg K, Campbell VL, Cavet J, Copland M, Searle E, Somervaille TCP
Cancer cell 2023 Dec 11;41(12):2136-2153.e13
Cancer cell 2023 Dec 11;41(12):2136-2153.e13
RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A.
Chen DB, Xie XW, Zhao YJ, Wang XY, Liao WJ, Chen P, Deng KJ, Fei R, Qin WY, Wang JH, Wu X, Shao QX, Wei L, Chen HS
Scientific reports 2020 Sep 3;10(1):14538
Scientific reports 2020 Sep 3;10(1):14538
Forkhead transcription factor FOXO3a mediates interferon-γ-induced MHC II transcription in macrophages.
Wu X, Fan Z, Chen M, Chen Y, Rong D, Cui Z, Yuan Y, Zhuo L, Xu Y
Immunology 2019 Dec;158(4):304-313
Immunology 2019 Dec;158(4):304-313
The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma.
Zhao Y, Xie X, Liao W, Zhang H, Cao H, Fei R, Wang X, Wei L, Shao Q, Chen H
Oncology reports 2017 Jan;37(1):289-296
Oncology reports 2017 Jan;37(1):289-296
In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport.
Dille S, Kleinschnitz EM, Kontchou CW, Nölke T, Häcker G
Infection and immunity 2015 Aug;83(8):3268-80
Infection and immunity 2015 Aug;83(8):3268-80
Interferon gamma repression of collagen (COL1A2) transcription is mediated by the RFX5 complex.
Xu Y, Wang L, Buttice G, Sengupta PK, Smith BD
The Journal of biological chemistry 2003 Dec 5;278(49):49134-44
The Journal of biological chemistry 2003 Dec 5;278(49):49134-44
The RFX family interacts at the collagen (COL1A2) start site and represses transcription.
Sengupta PK, Fargo J, Smith BD
The Journal of biological chemistry 2002 Jul 12;277(28):24926-37
The Journal of biological chemistry 2002 Jul 12;277(28):24926-37
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Hela Nuclear Extract. Load: 25 µg per lane. Primary antibody: RFX5 antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 65.3 kDa, 60 kDa for RFX5.
- Validation comment
- Western Blot
