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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- GTX107950 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107950, RRID:AB_10730232
- Product name
- ZAP70 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZAP70 antibody detects ZAP70 protein by Western blot analysis.A. 30 ?g Jurkat whole cell lysate/extract 7.5 % SDS-PAGEZAP70 antibody (GTX107950) dilution: 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ZAP70 antibody (GTX107950) diluted at 1:3000 and competitor's antibody (SC-574) diluted by 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ZAP70 antibody (GTX107950) diluted at 1:3000 and competitor's antibody (SC-574) diluted by 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZAP70 antibody detects ZAP70 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZAP70 protein stained by ZAP70 antibody (GTX107950) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZAP70 antibody immunoprecipitates ZAP70 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 ?g whole cell lysate/extract of ZAP70 protein expressing Jurkat cells B : Control with 3 ?g of pre-immune rabbit IgG C : Immunoprecipitation of ZAP70 by 3 ?g of ZAP70 antibody (GTX107950) 7.5% SDS-PAGE The immunoprecipitated ZAP70 protein was detected by ZAP70 antibody (GTX107950) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ZAP70 protein from Jurkat whole cell extracts using 5 £gg of ZAP70 antibody (GTX107950).Western blot analysis was performed using ZAP70 antibody (GTX107950).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ZAP70(GTX107950) antibody at 1:500 dilution.
