Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Zap-70 (Tyr315) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 70 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ZAP70 pTyr315 using Phospho-ZAP70 pTyr315 polyclonal antibody (Product # PA5-36884) at a dilution of 1:500. Lane 1: Hela cell lysate treated with H2O2 (100uM, 15min), Lane 2: Mouse kidney tissue lysate, Lane 3: Rat kidney tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Zap-70 (Tyr315) in Lane 1: HeLa cell lysate treated with H2O2 (100 µM,15mins), Lane 2: mouse kidney tissue lysate, Lane 3: rat kidney tissue lysate. Samples were incubated with Phospho-Zap-70 (Tyr315) polyclonal antibody (Product # PA5-36884) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-Zap-70 (Tyr315) in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-Zap-70 (Tyr315) polyclonal antibody (Product # PA5-36884) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.