Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 683702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#683702, RRID:AB_2572026
- Product name
- Purified anti-ZAP70 Phospho (Tyr319)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1503310
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cells were treated with 2 mM H2O2 for 15 minutes. Protein (15 µg) extracted from Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ZAP70 Phospho (Tyr319) (clone 1503310) antibody. Proteins were visualized using a HRP Goat anti-mouse IgG (minimal x-reactivity) antibody and chemiluminescence detection.
- Conjugate
- Unconjugated